Fig. 2

Mosaic BRE::gfp transcriptional activity in midgestation mouse hindbrain vasculature. Immunodetection of Endomucin and GFP in BRE::gfp embryos of 22 s (a–c) and 30s (d–f). Arrowheads indicate the heart region. Boxed areas represent the head region (b, e) and the intersomitic vessels (c, f). g Overview of a 22 s dorsal vascular plexus (Endomucin and GFP) in a flat-mounted hindbrain roof. Arrows indicate direction of rostral (R) to the left, caudal (C) to the right. Boxed areas (bottom panels) show sprouts at the midline. Asterisks depict GFP-positive tip cells. h Depth coding of a 30s dorsal vascular plexus and the original picture (right panel). Blue and green colours represent more ventral and dorsal tissues respectively. The vascular plexus at 30s consists of a superficial (i) and deeper plexus (j). Boxed areas are enlarged in the right panels. Scale bars: 500 μm (a; d); 100 μm (b–c; e–f); 75 μm (g, top; h; i–j, left panels); 50 μm (i–j, right panels); 25 μm (g, bottom)