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| | Vmem | pHi |
|---|
| Gradients | Fraction of S10B follicles | Fraction of S10B follicles |
| transversal§ | with FE2/FE1 ≥ 1.5 | with FE2/FE1 ≥ 1.5 |
| wt | 5/7 | 4/7 |
| grk | 1/7 | 0/7 |
| a-p# | with pFE/aFE ≥ 1.5 | with pFE/aFE ≥ 1.5 |
| wt | 2/7 | 4/7 |
| grk | 4/7 | 6/7 |
- §Transversal gradients (fluorescence intensity ratios FE2/FE1; larger value vs. smaller value), and #a-p gradients (fluorescence intensity ratios pFE/aFE) in the S10B FE of wt and grk were quantified as shown in Fig. 1e (for variability see Additional file: Tables S1 and S2). The higher the fluorescence intensity ratio is, the steeper is the gradient (n = 7; see als Fig. 3b and c). Since, in grk, the cFC and mFC (refering to aFE) are often both more hyperpolarised and more acidic than the aberrant pFC, a-p gradients in grk seem to be somewhat steeper than in wt (cf. Additional file: Fig. S1 and Table S2), but this difference was not significant (cf. Fig. 3c)
