Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools: tissue-specific expression of sensors for membrane potential and intracellular pH, and RNAi-knockdown of mechanisms involved in ion exchange

Table 1 Genes of ion-transport mechanisms and gap-junction subunits showing effects following RNAi-knockdown

Stock ID	Gene name	Protein function	Phenotype Soma	Germline
Proton pumps
VDRC46553	vha55	V-type H⁺-ATPase subunit	size-reduced ovaries, degeneration > S10A	not determined
BL40884	vha55		no effects	S11-S14 with NC rests, S14 with excess of FC around micropyle^b
Sodium channels
BL39053	rpk (dGNaC1)	DEG/epithelial sodium channel	no ovaries	small ovaries, ovarioles w/o follicles^a, S11-S14 with NC rests, S14 with excess of FC around micropyle
Potassium channels
BL53994	ork1	Open-rectifier potassium channel	spherical follicles (resembling kugelei mutant), alterations of basal cytoskeleton in FC (bMF & MT)	no effects^ab
Innexins
BL44048	inx1	Gap-junction subunit	no ovaries	S14 with NC-anomalies^a, many degenerating follicles^b
BL60112	inx3	Gap-junction subunit	small ovaries, no ovarioles, few follicles	no effects^ab

At least 10 females were scored for each strain
BL Bloomington Drosophila Stock Center number, VDRC Vienna Drosophila Resource Center number
Soma driver: tj-Gal4, ^a germline driver: MTD-Gal4, ^b germline driver: mat-tub-Gal4

ISSN: 1471-213X